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KMID : 1149220220300040281
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Korean Journal of Oriental Medical Prescription
2022 Volume.30 No. 4 p.281 ~ p.291
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Evaluation of Acute Toxicity of Pomace Schisandra chinensis Extracts Using SD-rats
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Kim Seok-Ho
Yoo Bo-Ra
Kim Young-Suk
Lim Jong-Min
Ku Bon-Hwa
Kwak Kyeung-Tae
Jeon Byeong-Yeob
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Abstract
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Objectives : In this study, acute oral toxicity test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis.
Methods : Pomace Schisandra chinensis extracts were orally administered to SD-rats(female, n=3) without a control group according to the 'OECD guidelines'. After, mortality and clinical signs were observed, and the deceased animals were subjected to an autopsy. In addition, acute oral toxicity test was sequentially performed in step Ⅰ(300 mg/kg), step Ⅱ(300 mg/kg), step Ⅲ(2,000 mg/kg), and step Ⅳ(2,000 mg/kg) according to the mortality.
Results : There were no abnormalities caused by pomace Schisandra chinensis extracts in step Ⅰ and step Ⅱ. However, one animal each died in step Ⅲ and step Ⅳ. In addition, clinical signs(salivation, decrease in food intake, prone position, decrease of locomotor activity, loss of locomotor activity, convulsion, hypothermia, lacrimation, staining around mouth, soiled perineal region, reddish urine, chromaturia, decrease of fecal volume, lying on side, blackish stool, no stool, compound-colored stool, refusal to feed, excitement, hypersensitivity, rigidity, dorsal position, etc.) were observed. But, no clinical signs were observed from 5th day, and experiment animals recovered completely.
Conclusions : As a result of this study, pomace Schisandra chinensis extracts may exhibit acute toxicity at concentrations of 2,000 to 5,000 mg/kg, and the GHS classification was designated as 'Category 5'.
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KEYWORD
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Pomace Schisandra chinensis, up-cycling, acute toxicity, OECD, GHS
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